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PCR Systems
Bio-Rad PCR Technology: Complete Beginner's Guide
This comprehensive beginner's guide explores Bio-Rad's PCR technology, focusing on the C1000 Touch Thermal Cycler and CFX96 Touch Real-Time PCR Detection System. It covers technical specifications, practical applications, and critical safety protocols for laboratory professionals. By understanding the advanced thermal cycling features and software interfaces of Bio-Rad instruments, users can optimize PCR workflows for diverse molecular biology applications. The guide includes detailed data tables and references to ensure reliable and efficient use of Bio-Rad PCR equipment.
Bio-Rad Equipment Authority
June 1, 2024
12 minutes
Technical Data
Bio-Rad C1000 Touch Thermal Cycler Technical Specifications
| Specification | Description |
|---|---|
| Block Formats | 96-well, 384-well, 48-well |
| Temperature Range | 4°C to 99°C |
| Temperature Accuracy | ±0.25°C |
| Ramp Rate | Up to 5°C/sec |
| Gradient Range | 30°C to 100°C |
| Maximum Volume per Well | 100 µL |
Bio-Rad CFX96 Touch Real-Time PCR System Specifications
| Specification | Description |
|---|---|
| Detection Channels | 5 (FAM, HEX, ROX, Cy5, Texas Red) |
| Block Format | 96-well plate |
| Temperature Range | 4°C to 100°C |
| Temperature Accuracy | ±0.2°C |
| Ramp Rate | 5°C/sec |
| Sensitivity | Detects as low as 10 copies/sample |
⚠️ Safety Protocols
- 1Verify that power cords and outlets comply with local electrical standards to prevent electrical hazards.
- 2Use heat-resistant gloves when handling PCR tubes immediately after runs to avoid thermal burns.
- 3Wear appropriate personal protective equipment when handling PCR reagents and dispose of chemical waste properly.
- 4Operate UV transilluminators with protective shields and eyewear to prevent UV exposure injuries.
- 5Perform regular cleaning and calibration of PCR instruments as recommended by Bio-Rad to maintain performance and safety.
References
- 1. Bio-Rad Laboratories. (2023). C1000 Touch Thermal Cycler User Manual. Retrieved from https://www.bio-rad.com
- 2. Bio-Rad Laboratories. (2023). CFX96 Touch Real-Time PCR Detection System Technical Bulletin. Retrieved from https://www.bio-rad.com
- 3. Mullis, K., & Faloona, F. (1987). Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods in Enzymology, 155, 335-350.